Effect of Ultraviolet - C Light Irradiation on the Bioburden of Hospital Contact Surfaces
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Microbial species replicate and propagate on every surface. Contact surfaces have become inevitable in hospitals as they are for convenience of staff and visitors. These hospital contact surfaces are microbial reservoirs, which play a leading role in the spread of nosocomial infections. Cleaning and disinfection of these surfaces deplete its bioburden, however, paucity of fund occasioned by lean healthcare budget has impacted on the continuous Cleaning and disinfection of these surfaces. We used a sterile swab moistened in normal saline to collect 600 samples in duplicate from various contact surfaces of 20 randomly selected hospitals before and after ultraviolet – c light irradiation. Standard microbiological assay were used to identify and characterize the isolated microbes. The mean of the pre-exposure bioburden in cfu/square swabbed surface was significantly higher than that of the post-exposure bioburden in cfu/square swabbed contact surface with (p ˂ 0.005). This result shows that ultraviolet – c light irradiation if effective in depleting the bioburden of hospital contact surfaces, we therefore, suggest the deployment of ultraviolet – c light irradiation in the integrated and continuous hospital contact surfaces disinfection regime.
Huslage, K., Rutala, W., Sickbert-Bennett, E., Weber, D. (2010) A Quantitative Approach to Defining “High – Touch” Surfaces in Hospital. Infect. Control Hosp. Epidemiol.;31:850 – 853.
Reckhow, D. A., Linden, K. G., Kim, J., Shemer, H. & Makdissy, G. (2010). Effect of UV treatment on DBP formation. J. Am. Water Works Assoc. 102, 100–113
Creamer E., Shore A., Deasy E., Galvin S., Dolan A., Walley N., et al(2021) Air and Surface Contamination Patterns of Methicillin-Resistant Staphylococcus aureus on Eight Acute Hospital Wards. J. Hosp. Infect. 86:201 – 208.
Okafor, S. A., Ekuma, I. C., Okey-Mbata, C. C., Ezeamaku, U. L., Okafor, A. L., Arukalam,
F. M., and Eziefuna, E. O. (2022), Investigating The Bioburden Of “Neglected” Hospital Low Contact Surfaces Advances in Microbiology., (12)5, 01 – 09.
Otter, J., French, G. (2009) Survival of Nosocomial Bacteria and Spores on Surfaces and Inactivation by Hydrogen Peroxide Vapour. J. Clin. Microbiol. 47, 205 – 20.
Kramer A., Schwebke I., Kampf G. (2006) How Long do Nosocomial Pathogens Persist on Inanimate Surfaces? A systematic Review. BMC Infect. Dis. 6,130.
Lee, F.Y., Mossad, S. B., Adal, K. A. (2020). "Pulmonary Mucormycosis: the Last 30 Years". Arch. Intern. Med. 159(12), 1301 - 09.
Mazza, P., Zani, F., Martelli, P. (2019) Studies on the antibiotic resistance of Bacillus subtilis Strains used in oral Bacteriotherapy. Boll. Chim. Farm. 131,401– 408.
Bhalla, A., Pultz, N. J., Gries, D. M., Ray, A. J., Eckstein, E. C, Aron, D. C. et al. (2004) Acquisition of Nosocomial Pathogens on Hands after Contact with Environmental Surfaces near Hospitalised Patients. Infect. Control Hosp. Epidemiol. 25, 164 – 67.
Dancer, S. J. (2009) The Role of Environmental Cleaning in The Control of Hospital- acquired Infection. J. Hosp. Infect. 73, 578 – 85.
Hayden, M. K., Bonten, M. J. M., Blom, D. W., Lyle, E.A, van de Vijver, D. A., Weinstein, R. A. (2006) Reduction in Acquisition of Vancomycin-resistant Enterococcus after Enforcement of Routine Environmental Cleaning Measures. Clin. Infect. Dis. 42(11), 1552 –60.
Okafor, S. A., Okey-Mbata, C. C., Daniel, J. A. Arukalam, F. M., Daniel-Nwosu E. I. and Okafor, A. L. (2021) Miscellany of Hospital Contact Surfaces Microbiome: A Case Study of Selected Hospitals in Owerri South Eastern Nigeria, Afr. .J Med. Phy., Biomed. Eng. & Sc., (8)2, 48 – 57.
Hayes, S. L., Sivaganesan, M., White, K. M. & Pfaller, S. L. (2008). Assessing the effectiveness
of low-pressure ultraviolet light for inactivating Mycobacterium avium complex (MAC) micro-organisms. Lett. Appl. Microbiol. 47, 386–392.
Kundrapu, S., Sunkesula, V., Jury, L. A., Sitzlar, B.M., Donskey, C. J. (2012) Daily Disinfection of High-touch Surfaces in Isolation Rooms to Reduce Contamination of Healthcare Workers’ Hands. Infect. Control Hosp. Epidemiol. 33(10), 1039 - 42.
Dancer, S. J., White, L .F., Lamb, J., Girvan, K. E., Robertson, C. (2009) Measuring the Effect of Enhanced Cleaning in a UK Hospital: A Prospective Cross - Over Study. BMC Med. 7,(1).
Bogusz, A., Stewart, M., Hunter, J., Yip, B., Reid, D., Robertson, C., Dancer, S J. 2013) How Quickly do Hospital surfaces become Contaminated after Detergent Cleaning? Healthc. Infect. 18(1), 3 – 9.
Riggs, M., Sethi, A., Zabarsky, T., Eckstein, E., Jump, R., Donskey, C. (2007) Asymptomatic Carriers are a Potential Source for Transmission of Epidemic and Nonepidemic Clostridium difficile Strains Among long-term care Facility Residents. Clin. Infect. Dis. 4, 992 – 98.
Christensen, J. & Linden, K. G. (2003). How particles affect UV light in the UV disinfection of
unfiltered drinking water. J. Am. Water Works Assoc. 95, 179–189.
[19] Ben Said, M., Masahiro, O. & Hassen, A. (2010). Detection of viable but non cultivable Escherichia coli after UV irradiation using a lytic Qβ phage. Ann. Microbiol. 60, 121–127.
Pullerits, K., Ahlinder, J., Holmer, L. et al. (2020). Impact of UV irradiation at full scale on bacterial communities in drinking water. npj Clean Water 3, 11 . https://doi.org/10.1038/s41545-020-0057-7.
Uwakwe, F. E, Ezejiofor, T. I. N., Ogbulie, T. E., Anyalogbu, E. A. A and Okafor, S. A (2023)
Investigating the Biodegradation of Low-Density Polyethylene by Proteus and Serratia
spp. Engineering and Technology Journal 8; 3, 2050-2055.
Bergevin, M., Marion, A., Farber, D., Golding, G. R., Levesque, S. (2017) Severe
MRSA Enterocolitis Caused by a Strain Habouring Enterotoxins D, G and I. Emerg. Infect. Dis. May 23(5), 865 – 67.
Sobel, J. D. (2007) Vulvovaginal candidosis. Lancet. 369,1961 - 1971.
Gehanno, J. F., Louvel, A., Nouvellon, M., Caillard, J. F., Pestel-Caron, M. (2020) Aerial Dispersal of Methicillin-resistant Staphylococcus aureus in Hospital Rooms by Infected or Colonized Patients. J. Hosp. Infect. 71, 256 – 62.
Wilson, A. P. R., Smyth, D., Moore, G., Singleton, J., Jackson, R., Gant, V., Jeanes, A.,
Shaw, S., James, E., Cooper, B., Kafatos, G., Cookson, B., Singer, M.,Bellingan, G. (2011) The Impact of Enhanced Cleaning Within the Intensive Care Unit on Contamination of the Near-patient Environment With Hospital Pathogens: a Randomized Crossover Study in Critical Care Units in Two Hospitals. Crit. Care Med. 39(4), 651 – 58.